In June 1989, 14-year-old Stephanie Isaacson headed out on her morning walk to Eldorado High School. The Las Vegas teenager frequently took a shortcut through a vacant sandlot off Steward Avenue and Linn Lane to get to school quicker. After school let out that afternoon, Stephanie’s father began to worry when she did not arrive home at her usual time. Upon calling the school in hopes of finding out where she may be, it was revealed that Stephanie never made it to school. A missing person report was filed and law enforcement investigators then conducted a local search, canvassing the area around the typical route that Stephanie would take to school. Her body was found at a sandlot and investigators determined she had been sexually assaulted and strangled to death. Over the years, a lengthy investigation, spanning decades, ensued and all available investigative leads were exhausted. Stephanie's killer remained undiscovered.
In 2021, the Las Vegas Metropolitan Police Department teamed up with Othram to reexamine DNA evidence from the crime scene in hopes that advanced DNA testing might generate new leads in the investigation. Funding assistance was generously provided by Justin Woo, founder of the non-profit organization Vegas Helps.
Othram scientists used Forensic-Grade Genome Sequencing® to build a genealogical profile from the remaining DNA evidence — only 120 picograms (or 0.12 nanograms) of DNA. This sets a new lower limit on the quantity of DNA required to build a genealogical profile for a suspect of a crime. The Othram genealogy team used the profile to develop investigative leads that were returned to LVMPD. LVMPD detectives were able to confirm the identity of the suspect in Stephanie's sexual assault and murder as Darren Roy Marchand. The suspect died in 1995 by suicide but has been tied to other crimes.
About Othram Inc.
Othram is the world’s first private DNA laboratory built specifically to apply the power of modern parallel sequencing to forensic evidence. Othram’s scientists are experts at recovery, enrichment, and analysis of human DNA from trace quantities of degraded or contaminated materials. Founded in 2018, and located in The Woodlands, Texas, our team works with academic researchers, forensic scientists, medical examiners, and law enforcement agencies to achieve results when other approaches fail. Follow Othram on Twitter @OthramTech or visit Othram.com to learn how we can help you with your case. Visit dnasolves.com to learn how anyone can make a difference in helping solve the next cold case.